Available Instruments and Services
Phenotype and transgene expression analysis
The Laboratory for Physiology and Genetics of Aquatic Organisms includes microscopy and microinjection facilities that allow for the production and analysis of transgenic fish, selective inactivation of gene function with morpholino oligonucleotides, as well as morphological analyses. Five microinjection stations allow the delivery of nucleic acids to fertilized zebrafish eggs. The facility contains a Nikon SMZ1500 microscope equipped for fluorescence and image analysis. Four additional stereomicroscopes are used for morphological examination of zebrafish embryos. The Laboratory is also equipped with a laser system to specifically ablate cells or to activate transgenes, and an electroporation system to transfect gene constructs and morpholinos into adult tissues.
The Laboratory maintains a variety of transgenic zebrafish that are available to all users within and outside the Faculty and University. For a full list of available transgenics, please see the Zebrafish website [coming soon].
Areas for surgery
These areas are shared by all users. They include a custom-built table for carrying out surgery on anesthetized fish. For physiological experiments, 6 wet tables, on which experimental chambers can be placed, are supplied with water and provided with adjustable gas mixtures.
Separate from the main facility is a closed system of approximately 100 tanks dedicated to imported zebrafish and those being used in experiments involving chemical or environmental exposures.
Microscopic imaging and electrophysiological recording systems
As an extension of the Laboratory, there are three additional facilities housed in D’Iorio, Gendron and CAREG that support microscopy, imaging and electrophysiological recording.
A major role of the Laboratory is to provide training to all users of advanced equipment for research on aquatic organisms. Laboratory technician, Vishal Saxena, has trained numerous students and postdoctoral fellows, and is available for training sessions in the following techniques:
- Zebrafish breeding and husbandry
- Microinjection techniques of nucleic acids or morpholinos into fertilized zebrafish embryos
- Fluorescence microscopy and image collection
If you are interested in a training session, and are a trainee affiliated with one of the primary users (i.e. professors) of the facility, please contact Vishal Saxena.